PHAGOCYTOSIS AND ANTI CLOTTING

 

PHAGOCYTOSIS (Neutrophil response during inflammation)

Definition: The process of engulfing of macromolecules like bacteria, dead cells & particulate matter by neutrophils, monocytes & tissue macrophages is called phagocytosis.

Steps involved in the process

    Margination: Neutrophils slow down in the circulation & get attached to the capillary wall. The

                           cell adhesion molecules adherin, Integrin & selectin are involved.

    Diapedesis: Neutrophils squeeze through the pores in the capillary wall into the tissues.

    Chemotaxis : Neutrophils are attracted towards the site of damage ( infection & inflammation)

                            by the chemicals released at the site of damage.

     Engulfing :

                  Microbes are coated with complement & antibodies (opsonization)

                                                              

                       Neutrophil membrane invaginates enclosing the microbe

                                                               

                                               Formation of vacuole

                                                                

                                          Vacuole fusing with lysosome

                                                                                                                                                        

Lysosomes release hydrolyzing enzymes & bactericidal agents like hydrogen peroxide, mycloperoxidase etc

                                                                

                          

                   Killing & digestion of the microbe.  

                          4. ANTICLOTTING MECHANISMS (Factors that prevent intravascular coagulation)

1. Physical characteristics of the endothelium (e.g) Smoothness of vascular endothelium Atherosclerotic (deposit of fat) plague in the vascular endothelium gives a rough surface which activates platelets & initiate clotting.

2. High blood flow rates: Increased velocity of blood flow prevents clotting.

3.Presence of natural anticoagulants: Anticoagulants like heparin, anti thrombin III & α2macro globulin are some of the natural anti coagulants present in the circulation and prevent the intra vascular clotting .

4. Fibrinolytic system: When small clots are formed in the vessels they are immediately lysed by the system.

              Tissue Plasminogen Activator (tPA)

                                            

               Plasminogen     → Plasmin (digests the fibrin into soluble fragments dissolving the clot)

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